Premium
Membrane association of FtsY, the E. coli SRP receptor
Author(s) -
de Leeuw Erik,
Poland Dennis,
Mol Olaf,
Sinning Irmgard,
ten Hagen-Jongman Corinne M,
Oudega Bauke,
Luirink Joen
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01238-6
Subject(s) - cytoplasm , gtp' , subcellular localization , escherichia coli , signal recognition particle receptor , microbiology and biotechnology , biology , biochemistry , membrane , chemistry , biophysics , membrane protein , protein targeting , gene , enzyme
FtsY, the Escherichia coli homologue of the eukaryotic SRP receptor (SRα), is located both in the cytoplasm and in the inner membrane of E. coli . Similar to SRα, FtsY consists of two major domains: a strongly acidic N‐terminal domain (A) and a C‐terminal GTP binding domain (NG) of which the crystal structure has recently been determined. The domains were expressed both in vivo and in vitro to examine their subcellular localization. The results suggest that both domains associate with the membrane but that the nature of the association differs.