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Organization and expression of the chum salmon insulin‐like growth factor II gene
Author(s) -
Palamarchuk Alexey Y.,
Holthuizen P.Elly,
Müller Werner E.G.,
Sussenbach John S.,
Kavsan Vadim M.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01217-9
Subject(s) - gene , biology , exon , gene expression , transcription factor , signal peptide , amino acid , promoter , growth factor , microbiology and biotechnology , peptide sequence , genetics , receptor
IGF‐II plays an important role in growth and development of vertebrates. In the present study, the characterization of the first fish IGF‐II gene, chum salmon IGF‐II, is described. The sIGF‐II gene consists of four exons, spanning a region of 9 kbp, that encode the 214 aa IGF‐II precursor. While the amino acid sequences of fully processed IGF‐II of salmon and mammalian species are very similar, the prepro‐peptide sequence deviates extensively in the signal‐ and E‐peptide domains. The transcription initiation site of the sIGF‐II gene was localized within a 30 nt region employing RT‐PCR. Using sIGF‐II promoter‐luciferase constructs it was demonstrated that the sIGF‐II gene has a relatively strong promoter that contains tissue‐specific regulatory elements.