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Definitive chemical evidence for the constitutive ability of Candida albicans serotype A strains to synthesize β‐1,2 linked oligomannosides containing up to 14 mannose residues
Author(s) -
Trinel P.A.,
Lepage G.,
Jouault T.,
Strecker G.,
Poulain D.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01205-2
Subject(s) - candida albicans , mannose , serotype , microbiology and biotechnology , chemistry , biology , stereochemistry , biochemistry
We have previously reported the presence of phosphate bound β‐1,2 linked oligomannosides with unusually high degrees of polymerization (DP>7) in the mannan of Candida albicans strain VW32. To confirm this observation, we have prepared these oligomannosides from the mannan of C. albicans strain NIH A 207. Gel filtration chromatography and TLC analysis revealed DP up to 14. For both strains, NMR analysis confirmed the exclusive presence of β‐1,2 linkages in the pools of oligomannosides with a DP higher than 6 which presented an average DP of 10.6 (VW32) and 10.4 (NIH A 207). These results are important to consider in relation with the ability of these C. albicans derived oligomannosides to trigger TNFα synthesis according to their DP.