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Trifluoroethanol‐induced conformational transition of hen egg‐white lysozyme studied by small‐angle X‐ray scattering
Author(s) -
Hoshino Masaru,
Hagihara Yoshihisa,
Hamada Daizo,
Kataoka Mikio,
Goto Yuji
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01172-1
Subject(s) - radius of gyration , lysozyme , small angle x ray scattering , scattering , chemistry , circular dichroism , gyration , crystallography , small angle scattering , grazing incidence small angle scattering , small angle neutron scattering , polymer , neutron scattering , optics , physics , organic chemistry , geometry , biochemistry , mathematics
The trifluoroethanol (TFE)‐induced conformational transition of hen lysozyme was studied with the combined use of far‐UV circular dichroism (CD) and small‐angle X‐ray scattering. At pH 2.0 and 20°C, the addition of TFE to the native lysozyme induced a cooperative transition to an intermediate state with an increased helical content (TFE state). Small‐angle X‐ray scattering measurements indicated that the TFE state has a radius of gyration which is 20% larger than that of the native state and assumes a chain‐like conformation with some remaining globularity. The TFE‐induced transition curves obtained by CD and the small‐angle X‐ray scattering measurements agreed well, consistent with a two‐state transition mechanism. A singular value decomposition analysis of Kratky plots of the small‐angle X‐ray scattering profiles indicated that two basic scattering functions reproduce the observed spectra, further confirming the validity of a two‐state approximation.