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Role of glutathione and nitric oxide in the energy metabolism of rat liver mitochondria
Author(s) -
Nishikawa Manabu,
Sato Eisuke F.,
Kuroki Tetsuo,
Inoue Masayasu
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01156-3
Subject(s) - glutathione , mitochondrion , nitric oxide , respiration , cytosol , buthionine sulfoximine , chemistry , biochemistry , metabolism , oxidative phosphorylation , biology , enzyme , anatomy , organic chemistry
Previous studies have suggested that nitric oxide (NO) inhibited mitochondrial respiration. NO and/or its intermediate(s) react with various molecules, such as hemeproteins and free SH groups. The inhibitory effect of NO on mitochondrial respiration was decreased by exogenously added glutathione (GSH). However, a decrease of intramitochondrial GSH by pretreating animals with l ‐buthionine sulfoximine had no appreciable effect on the inhibitory effect of isolated mitochondria. Furthermore, the effect of NO was not affected by depleting free SH residues in mitochondria by N ‐ethylmaleimide. These results suggest that cytosolic but not intramitochondrial GSH might be an important factor that determines the NO‐dependent regulation of mitochondrial energy metabolism.