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Inhibition of DNA synthesis and tube morphogenesis of cultured vascular endothelial cells by chondromodulin‐I
Author(s) -
Hiraki Yuji,
Kono Takaharu,
Sato Mutsumi,
Shukunami Chisa,
Kondo Jun
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01151-4
Subject(s) - cartilage , in vitro , microbiology and biotechnology , morphogenesis , chemistry , dna synthesis , glycoprotein , fetus , anatomy , biology , biochemistry , genetics , gene , pregnancy
Cartilage is an avascular tissue, and exhibits anti‐angiogenic properties. Cartilage extracts have been shown to contain an inhibitor for DNA synthesis in vascular endothelial cells in vitro. Here we purified the inhibitory activity in the 10–50 kDa fraction of guanidine extracts from fetal bovine epiphyseal cartilage, and found that the inhibitor was identical with chondromodulin‐I (ChM‐I). Purified ChM‐I inhibited tube morphogenesis of cultured vascular endothelial cells, as well as DNA synthesis. These results indicate that cartilage‐specific glycoprotein ChM‐I may participate in the maintenance of avascularity and anti‐angiogenic properties of cartilage.