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Cross‐linked trimers of bovine ribonuclease A: activity on double‐stranded RNA and antitumor action
Author(s) -
Gotte Giovanni,
Testolin Lucia,
Costanzo Chiara,
Sorrentino Salvatore,
Armato Ubaldo,
Libonati Massimo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01147-2
Subject(s) - rnase p , ribonuclease , bovine pancreatic ribonuclease , rna , chemistry , ribonuclease iii , cell culture , rnase h , microbiology and biotechnology , biochemistry , biology , genetics , gene , rna interference
Trimers of bovine pancreatic RNase A were obtained by cross‐linking native RNase A with dimethyl suberimidate. They degrade double‐stranded RNA more efficiently than dimers and monomers of RNase A, and display significant cytotoxic and/or cytostatic actions against C4‐I cells (a human cell line derived from squamous carcinoma of the uterus cervix). On the same cell line cross‐linked dimers of RNase A appear to be ineffective.