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HIV‐1 capsid mutants inhibit the replication of wild‐type virus at both early and late infection phases
Author(s) -
Furuta Rika A,
Shimano Reika,
Ogasawara Takashi,
Inubushi Ritsuko,
Amano Kazushi,
Akari Hirofumi,
Hatanaka Masakazu,
Kawamura Meiko,
Adachi Akio
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01132-0
Subject(s) - capsid , mutant , group specific antigen , virology , viral replication , biology , virus , wild type , gene , genetics
In‐frame mutations were introduced into various portions of the human immunodeficiency virus type 1 (HIV‐1) gag gene, and potentials of the mutants to suppress the replication of wild‐type HIV‐1 were monitored. In contrast to results obtained with matrix and nucleocapsid mutants, almost all capsid mutants blocked HIV‐1 replication completely in single‐round replication assays. A capsid mutant designated C6b was demonstrated to be one of the most efficient inhibitors for HIV‐1 reported to date, and to be effective at both early and late viral replication phases. T‐cells, which are engineered to express the C6b Gag in response to HIV‐1 infection, were perfectly resistant to HIV‐1.