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Sequential assignments and secondary structure of the RNA‐binding transcriptional regulator NusB 1
Author(s) -
Altieri Amanda S.,
Mazzulla Marie J.,
Zhou Hongjun,
Costantino Nina,
Court Donald L.,
Byrd R.Andrew
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01128-9
Subject(s) - regulator , rna , chemistry , transcriptional regulation , computational biology , biology , biochemistry , gene , gene expression
The NusB protein is involved in transcriptional regulation in bacteriophage λ. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg‐rich RNA‐binding motif (ARM) at the N‐terminus but is not sequentially homologous to any other proteins. In contrast to other known ARM‐containing proteins, NusB forms a stable structure in solution in the absence of RNA. NMR spectroscopy was used to determine that NusB contains six α‐helices: R10–Q21, I27–F34, V45–L65, Q79–S93, Y100–F114 and D118–L127. The structure of NusB makes it a member of a newly emerging class of α‐helical RNA‐binding proteins.