Premium
Preliminary NMR studies of Thermus thermophilus ribosomal protein S19 overproduced in Escherichia coli
Author(s) -
Davydova Natalia L,
Rak Alexey V,
Gryaznova Olga I,
Liljas Anders,
Jonsson Bengt-Harald,
Berglund Helena,
Härd Torleif,
Garber Maria B
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01112-5
Subject(s) - thermus thermophilus , escherichia coli , ribosomal protein , ribosomal rna , chemistry , biochemistry , microbiology and biotechnology , biology , ribosome , gene , rna
The gene for the ribosomal protein S19 from Thermus thermophilus was cloned, sequenced and overexpressed in Escherichia coli . A simple procedure for isolating the recombinant protein was developed. Preliminary NMR studies revealed a high content of α‐helical secondary structure in the protein.