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Inhibition of the alternative oxidase stimulates H 2 O 2 production in plant mitochondria
Author(s) -
Popov V.N,
Simonian R.A,
Skulachev V.P,
Starkov A.A
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01099-5
Subject(s) - alternative oxidase , mitochondrion , oxidase test , biochemistry , cyanide , propyl gallate , respiration , chemistry , oxidizing agent , salicylhydroxamic acid , enzyme , biology , botany , organic chemistry , antioxidant
The hypothesis that a non‐coupled alternative oxidase of plant mitochondria operates as an antioxygen defence mechanism [Purvis, A.C. and Shewfelt, R.L., Physiol. Plant. 88 (1993) 712–718; Skulachev, V.P., Biochemistry (Moscow) 59 (1994) 1433–1434] has been confirmed in experiments on isolated soybean and pea cotyledon mitochondria. It is shown that inhibitors of the alternative oxidase, salicyl hydroxamate and propyl gallate strongly stimulate H 2 O 2 production by these mitochondria oxidizing succinate. Effective concentrations of the inhibitors proved to be the same as those decreasing the cyanide‐resistant respiration. The inhibitors proved to be ineffective in stimulating H 2 O 2 formation in rat liver mitochondria lacking the alternative oxidase.