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The acetyl‐CoA synthetase gene ACS2 of the yeast Saccharomyces cerevisiae is coregulated with structural genes of fatty acid biosynthesis by the transcriptional activators Ino2p and Ino4p
Author(s) -
Hiesinger Margit,
Wagner Christian,
Schüller Hans-Joachim
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01085-5
Subject(s) - saccharomyces cerevisiae , biochemistry , upstream activating sequence , gene , biology , activator (genetics) , structural gene , yeast , biosynthesis , transcription factor , transcription (linguistics) , promoter , gene expression , mutant , linguistics , philosophy
The yeast Saccharomyces cerevisiae contains two acetyl‐CoA synthetase genes, ACS1 and ACS2 . While ACS1 transcription is glucose repressible, ACS2 shows coregulation with structural genes of fatty acid biosynthesis. The ACS2 upstream region contains an ICRE (inositol/choline‐responsive element) as an activating sequence and requires the regulatory genes INO2 and INO4 for maximal expression. We demonstrate in vitro binding of the heterodimeric activator protein Ino2p/Ino4p to the ACS2 promoter. In addition, the pleiotropic transcription factor Abf1p also binds to the ACS2 control region. The identification of ACS2 activating elements also found upstream of ACC1 , FAS1 and FAS2 suggests a role of this acetyl‐CoA synthetase isoenzyme for the generation of the acetyl‐CoA pool required for fatty acid biosynthesis.