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Rescue of a neutralizing anti‐viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells
Author(s) -
Gargano Nicola,
Cattaneo Antonino
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01065-x
Subject(s) - polyclonal antibodies , intracellular , antibody , recombinant dna , virology , microbiology and biotechnology , biology , neutralizing antibody , gene , immunology , genetics
The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this reason, it would be desirable to exploit intracellular expression of antibodies to select or rescue more efficient ones from a polyclonal mixture. In this work we have successfully explored this possibility by rescuing a neutralizing anti‐viral antibody fragment from an intracellularly expressed anti‐reverse transcriptase polyclonal repertoire.