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Chloroplastic prenylated proteins
Author(s) -
Parmryd Ingela,
Shipton Catherine Ann,
Swiezewska Ewa,
Dallner Gustav,
Andersson Bertil
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01063-6
Subject(s) - prenylation , thylakoid , chloroplast , thermolysin , cytoplasm , biochemistry , inner membrane , spinach , chloroplast membrane , membrane protein , biology , membrane , chemistry , enzyme , trypsin , gene
By in vivo [ 3 H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS‐PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.