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Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies
Author(s) -
Arbabi Ghahroudi M,
Desmyter A,
Wyns L,
Hamers R,
Muyldermans S
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01062-4
Subject(s) - panning (audio) , recombinant dna , immunoglobulin light chain , antigen , computational biology , antibody , biology , cloning (programming) , heavy chain , modular design , identification (biology) , single domain antibody , repertoire , microbiology and biotechnology , genetics , gene , computer science , botany , programming language , paleontology , zoom , lens (geology) , physics , acoustics
Functional heavy‐chain γ ‐immunoglobulins lacking light chains occur naturally in Camelidae . We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy‐chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli , extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins.