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Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells
Author(s) -
Nindl G,
Swez J.A,
Miller J.M,
Balcavage W.X
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01060-0
Subject(s) - jurkat cells , dna synthesis , dna , growth inhibition , thymidine , electromagnetic field , chemistry , biophysics , microbiology and biotechnology , cell growth , biology , immunology , biochemistry , physics , t cell , immune system , quantum mechanics
A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [ 3 H] thymidine uptake of the latter cells by almost 50% vs. 20–25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs were very similar suggesting saturation at 0.1 mT or lower.