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Pulsed EPR measurement of the distance between P 680 +· and Q A −· in photosystem II
Author(s) -
Stephan G. Zech,
Jens Kurreck,
Hann-Jörg Eckert,
Г. Ренгер,
Wolfgang Lubitz,
Robert Bittl
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01054-5
Subject(s) - p680 , photosystem ii , electron paramagnetic resonance , chemistry , spinach , electron transfer , p700 , hyperfine structure , electron acceptor , photosynthetic reaction centre , acceptor , analytical chemistry (journal) , photochemistry , photosystem i , nuclear magnetic resonance , photosynthesis , biochemistry , atomic physics , physics , condensed matter physics , chromatography
Out‐of‐phase electron spin echo envelope modulation (ESEEM) spectroscopy was used to determine the distance between the primary donor radical cation P 680 +· and the quinone acceptor radical anion Q A −· in iron‐depleted photosystem II in membrane fragments from spinach that are deprived of the water oxidizing complex. Furthermore, a lower limit for the distance between the oxidized tyrosine residue Y z of polypeptide D1 and Q A −· could be estimated by a comparison of data gathered from samples where the electron transfer from Y z to P 680 +· is either intact or blocked by preillumination in the presence of NH 2 OH.