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SNAP‐23 is located in the basolateral plasma membrane of rat pancreatic acinar cells
Author(s) -
Herbert Y. Gaisano,
Laura Sheu,
Pauline Wong,
Amira Klip,
William S. Trimble
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01013-2
Subject(s) - exocytosis , microbiology and biotechnology , epithelial polarity , secretion , munc 18 , vesicle , chemistry , snare complex , pancreatic islets , syntaxin , membrane , enteroendocrine cell , secretory vesicle , biology , endocrinology , islet , endocrine system , synaptic vesicle , biochemistry , insulin , hormone
The SNARE hypothesis proposes that specificity of exocytosis is regulated by the appropriate interactions between the vesicle (v‐) SNARE and the target membrane (t‐) SNAREs. We show here that pancreatic acinar cells express the SNAP‐25 t‐SNARE homolog SNAP‐23, and find that this t‐SNARE is most highly concentrated on the basolateral plasma membrane while being expressed below detectable levels in endocrine islets within the same tissue. This is the first localization of SNAP‐23 within a polarized tissue and suggests that this t‐SNAREs may interact with syntaxin‐4 to mediate basolateral secretion.