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Direct expression of PCR products in a cell‐free transcription/translation system: synthesis of antibacterial peptide cecropin
Author(s) -
Kirill A. Martemyanov,
Alexander S. Spirin,
A.T. Gudkov
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01011-9
Subject(s) - cecropin , transcription (linguistics) , cell free protein synthesis , dna , antibacterial peptide , peptide , translation system , plasmid , computational biology , cell free system , translation (biology) , biology , cloning (programming) , microbiology and biotechnology , chemistry , protein biosynthesis , antibacterial activity , messenger rna , bacteria , gene , biochemistry , genetics , computer science , in vitro , antimicrobial peptides , linguistics , philosophy , programming language
A simple and effective methodology is proposed for direct expression of PCR‐generated linear DNAs in cell‐free transcription/translation systems without cloning DNA fragments in plasmids. This methodology is realized for the synthesis of the active antibacterial peptide cecropin using the synthetic coding sequence. Possible scientific applications and perspectives of the proposed approach are discussed.