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Incorporation of 1‐deoxy‐ d ‐xylulose into isoprene and phytol by higher plants and algae
Author(s) -
Schwender Jörg,
Zeidler Johannes,
Gröner Rainer,
Müller Christian,
Focke Manfred,
Braun Siegmar,
Lichtenthaler Frieder W,
Lichtenthaler Hartmut K
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01002-8
Subject(s) - phytol , isoprene , terpenoid , chloroplast , algae , mevalonate pathway , mevalonic acid , photosynthesis , biosynthesis , botany , biochemistry , chemistry , biology , stereochemistry , organic chemistry , gene , copolymer , enzyme , polymer
In further substantiating the novel mevalonate‐independent pathway for isoprenoid biosynthesis, which generates isopentenyl diphosphate (IPP) via 1‐deoxy‐ d ‐xylulose‐5‐phosphate, labeling experiments with 1‐[ 2 H 1 ]deoxy‐ d ‐xylulose were performed with various higher plants and algae: efficient incorporation was observed into isoprene emitted by Populus , Chelidonium , and Salix , into the phytol moiety of chlorophylls in a red alga ( Cyanidium ), in two green algae ( Scenedesmus , Chlamydomonas ), and a higher plant ( Lemna ). By contrast, 13 C‐mevalonate applied was incorporated into isoprene and phytol to a much lower extent or not at all. This demonstrates that this `1‐deoxy‐ d ‐xylulose‐5‐phosphate pathway' for biosynthesis of plastidic isoprenoids is widely distributed in photosynthetic organisms.