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Different expression of adenylyl cyclase isoforms after retinoic acid induction of P19 teratocarcinoma cells
Author(s) -
Lipskaia Larissa,
Djiane Alexandre,
Defer Nicole,
Hanoune Jacques
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01001-6
Subject(s) - teratocarcinoma , retinoic acid , adenylyl cyclase , p19 cell , gene isoform , chemistry , microbiology and biotechnology , biology , biochemistry , cellular differentiation , gene , enzyme , adult stem cell
We have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid (RA)‐primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of neuroectodermal formation. Here we show that the P19 stem cells possess a basal Ca 2+ /CaM‐stimulated AC activity, which increases about 10‐fold after RA induction. The rise of AC activity is associated with a stage‐specific up‐regulation of AC2, AC5 and AC8 mRNAs and a down‐regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the role and specific regulation of AC isoforms during neuronal differentiation.