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Ceramide‐induced translocation of protein kinase C ζ in primary cultures of astrocytes
Author(s) -
Galve-Roperh Ismael,
Haro Amador,
Dı́az-Laviada Inés
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00985-x
Subject(s) - ceramide , protein kinase c , sphingomyelin , western blot , chromosomal translocation , microbiology and biotechnology , signal transduction , phosphorylation , intracellular , kinase , protein kinase a , marcks , biology , chemistry , biochemistry , apoptosis , membrane , gene
The present research was undertaken to study the possible involvement of the atypical protein kinase C (PKC) ζ in ceramide signal transduction in primary cultures of rat astrocytes. As shown by Western blot analysis, translocation of immunoreactive PKCζ to the particulate fraction occurred upon exposure of astrocytes to cell‐permeable ceramide analogs or to exogenous sphingomyelinase. The particulate fraction may correspond to a perinuclear area, as indicated by immunocytochemical techniques. Furthermore, treatment of cells with N ‐octanoylsphingosine led to an increased phosphorylation of PKCζ. Results thus show that stimulation of PKCζ may be one of the intracellular events triggered by activation of the sphingomyelin pathway.