Premium
The transcriptional co‐activator proteins p300 and CBP stimulate adenovirus E1A conserved region 1 transactivation‐independent of a direct interaction
Author(s) -
Mannervik Mattias,
Akusjärvi Göran
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00982-4
Subject(s) - transactivation , activator (genetics) , transcription (linguistics) , transcription factor , heterologous , microbiology and biotechnology , creb binding protein , promoter , dna binding protein , dna binding domain , chemistry , biology , gene , genetics , gene expression , linguistics , philosophy , creb
p300 and CBP are two related transcriptional co‐activator proteins required by many cellular transcription factors for activity. The adenovirus E1A protein binds p300 and CBP through its amino‐terminus and conserved region (CR) 1. Fusing CR1 to a heterologous DNA‐binding domain creates a potent transcriptional activator, suggesting that CR1 might activate transcription by recruiting p300/CBP to the promoter. We show that both p300 and CBP enhances CR1‐dependent transactivation. However, this enhancement occurs independently of a direct interaction with E1A and does not correlate with the CR1 activator function.