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Human serotonin 5‐HT 7 receptor: cloning and pharmacological characterisation of two receptor variants
Author(s) -
Stam Nico J,
Roesink Carolien,
Dijcks Fred,
Garritsen Anja,
van Herpen Anne,
Olijve Wiebe
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00964-2
Subject(s) - 5 ht receptor , cloning (programming) , serotonin , receptor , 5 ht5a receptor , chemistry , biology , microbiology and biotechnology , genetics , computer science , programming language
Two splice variants of the 5‐HT 7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT 7 receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH‐3T3 cells, both variants encode functional 5‐HT 7 receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5‐HT 7 receptor genes may exist in human.