Premium
Chinese hamster protein homologous to human putative protein kinase KIAA0204 is associated with nuclei, microtubules and centrosomes in CHO‐K1 cells
Author(s) -
Zinovkina Lyudmila A.,
Poltaraus Andrey B.,
Solovyanova Olga B.,
Nadezhdina Elena S.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00952-6
Subject(s) - chinese hamster ovary cell , centrosome , microbiology and biotechnology , biology , complementary dna , protein kinase a , microtubule , cdna library , kinase , gene , biochemistry , cell culture , genetics , cell cycle
Monoclonal antibody raised against a preparation of loach fish sperm centrosomes was used for screening of cDNA expressing library of Chinese hamster CHO‐K1 cells. Two positive clones appeared to encode 628 amino acid protein fragment that was 72% identical to human KIAA0204 protein, i.e. putative protein kinase. Polyclonal antibodies raised against products of cDNA expression in E. coli recognized 210‐kDa polypeptide in CHO‐K1 cells and immunostained nuclear speckles, centrosomes and microtubules in these cells. The 210‐kDa polypeptide (named MAK‐L) co‐sedimented with exogenous microtubules. Thus, one more protein kinase seems to be associated with the microtubule network in vertebrate cells.