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Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR , a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin
Author(s) -
Ortego Javier,
Escribano Julio,
Coca-Prados Miguel
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00934-4
Subject(s) - leucine zipper , ciliary body , homology (biology) , microbiology and biotechnology , gene , biology , cloning (programming) , peptide sequence , genetics , neuroscience , computer science , programming language
A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open angle glaucoma [Science 275 (1997) 668–670]. Several structural features of the deduced human protein have been noted: a cleavable N‐terminal signal peptide, a periodic repetition at the N‐terminus of leucine and arginine residues at every seventh and eleven position respectively in helix conformation (leucine zipper‐like motif) exhibiting homology with myosin, and with olfactomedin in the C‐terminus. The mRNA for TIGR is abundantly expressed in the ciliary body, iris, heart and skeletal muscle.