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Peroxynitrite‐induced hemolysis of human erythrocytes and its inhibition by antioxidants
Author(s) -
Kondo Hiroshi,
Takahashi Mareyuki,
Niki Etsuo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00922-8
Subject(s) - peroxynitrite , hemolysis , chemistry , trolox , ebselen , antioxidant , nitric oxide , methemoglobin , hemoglobin , biochemistry , glutathione , superoxide , superoxide dismutase , antioxidant capacity , glutathione peroxidase , immunology , organic chemistry , biology , enzyme
It was found that human erythrocytes underwent hemolysis when incubated with peroxynitrite at 37°C under air. The extent of hemolysis increased with increasing peroxynitrite concentration and decreasing hematocrit. The peroxynitrite‐induced hemolysis was suppressed only partially by a radical scavenging antioxidant such as uric acid and Trolox, a water‐soluble vitamin E analogue, but reduced glutathione, N ‐acetylcysteine and albumin efficiently inhibited the hemolysis. A selenium‐containing organic compound, ebselen, also suppressed the hemolysis. On the other hand, nitric oxide and superoxide generated concomitantly from 3‐morpholinosydnonimine (SIN‐1) did not induce appreciable hemolysis, while it converted hemoglobin to methemoglobin extensively.