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Possible involvement of radical reactions in desialylation of LDL
Author(s) -
Tanaka Kyoko,
Tokumaru Sadako,
Kojo Shosuke
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00917-4
Subject(s) - chemistry , sialic acid , scavenger , radical , conjugated system , oxidative phosphorylation , butylated hydroxytoluene , biochemistry , pathogenesis , lipid peroxidation , scavenger receptor , low density lipoprotein , antioxidant , cholesterol , medicine , organic chemistry , lipoprotein , polymer
The role of oxidatively modified LDL in the pathogenesis of atherosclerosis has been well documented. These studies have focused on modifications of lipid and protein parts of LDL. Recently desialylated LDL has received attention in relation to atherosclerosis and coronary artery disease. We examined the possible involvement of radical reactions in desialylation of LDL. Human LDL was subjected to oxidative damage using Cu 2+ ion. As the conjugated dienes monitored by absorption at 234 nm increased, the content of sialic acid decreased steadily. Both the elevation of conjugated diene and the decrease of sialic acid were inhibited by β‐mercaptoethanol, a typical radical scavenger. Besides, both butylated hydroxytoluene and a nitrogen atmosphere inhibited the decrease of sialic acid. These inhibition experiments suggested that sialic acid moieties in LDL were reactive toward radicals.