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Annexin I targets S100C to early endosomes
Author(s) -
Seemann Joachim,
Weber Klaus,
Gerke Volker
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00911-3
Subject(s) - endosome , annexin a2 , microbiology and biotechnology , annexin , chemistry , cell fractionation , subcellular localization , membrane , biology , receptor , biochemistry , cytoplasm , in vitro
Immunofluorescence and subcellular fractionation localize annexin I and the EF hand protein S100C to the same membranous structures which in part correspond to transferrin receptor‐positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop–S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to co‐localize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.