Premium
Distinct type‐2A protein phosphatases activate HMGCoA reductase and acetyl‐CoA carboxylase in liver
Author(s) -
Gaussin Vinciane,
Skarlas Polymnia,
Ching Yick Pang,
Hardie D.Grahame,
Hue Louis
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00890-9
Subject(s) - pyruvate carboxylase , phosphatase , reductase , biochemistry , protein kinase a , enzyme , chemistry , hmg coa reductase , acetyl coa carboxylase , biology
Acetyl‐CoA carboxylase and HMGCoA reductase are inactivated by the same AMP‐activated protein kinase and are activated by type‐2A protein phosphatase. To determine whether the same species of protein phosphatase‐2A were involved, we studied the interconversion of acetyl‐CoA carboxylase and HMGCoA reductase in isolated rat hepatocytes. We show that (i) these enzymes are differently regulated in hepatocytes and (ii) the species of type‐2A protein phosphatase involved in their activation are different and can be separated by anion‐exchange chromatography.