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Inhibitors of poly(ADP‐ribose) polymerase suppress nuclear fragmentation and apoptotic‐body formation during apoptosis in HL‐60 cells
Author(s) -
Shiokawa Daisuke,
Maruta Hideharu,
Tanuma Sei-ichi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00887-9
Subject(s) - poly adp ribose polymerase , dna fragmentation , apoptosis , fragmentation (computing) , apoptotic dna fragmentation , chromatin , polymerase , microbiology and biotechnology , prophase , apoptotic body , chemistry , benzamide , dna , biology , biochemistry , programmed cell death , stereochemistry , gene , ecology , meiosis
The effects of 3‐aminobenzamide (3ABm) and benzamide (BAm), known specific inhibitors of poly(ADP‐ribose) polymerase (PARP), on actinomycin D (Act D)‐induced apoptosis in HL‐60 cells were examined. These inhibitors had no appreciable effect on apoptotic DNA fragmentation, chromatin condensation or PARP restriction cleavage, but clearly inhibited morphological changes, especially nuclear fragmentation and apoptotic‐body formation, in a dose‐dependent manner. These results suggest that the synthesis of ADP‐ribose polymers is not essential for the progression of apoptotic DNA fragmentation and chromatin condensation, but is required in the processes leading to nuclear fragmentation and the subsequent apoptotic‐body formation during apoptosis in HL‐60 cells.