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HIV‐1 envelope protein, gp120, has no effects on inositol phosphate production and metabolism in the Jurkat T‐cell line either in the presence or absence of receptor stimulation
Author(s) -
Sumner Martina T,
Shears Stephen B
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00880-6
Subject(s) - jurkat cells , inositol , stimulation , inositol phosphate , metabolism , chemistry , receptor , microbiology and biotechnology , biochemistry , t cell , biology , endocrinology , immunology , immune system
We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6‐1 CD4 + T‐cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor‐activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol polyphosphate 5‐phosphatase. Treatment of cells with up to 10 μg/ml gp120 from between 10 min and 24 h was without effect upon inositol phosphate turnover in both basal cells, and in C305 and OKT3 stimulated cells. This is the first report that biologically competent gp120 does not affect any aspect of inositol phosphate turnover in either basal or receptor‐activated lymphocytes.