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Over‐expression of plant 14‐3‐3 proteins in tobacco: enhancement of the plasmalemma K + conductance of mesophyll cells 1 2
Author(s) -
Saalbach Gerhard,
Schwerdel Marc,
Natura Gabriel,
Buschmann Peter,
Christov Veselin,
Dahse Ingo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00865-x
Subject(s) - complementary dna , vicia faba , protoplast , guard cell , amino acid , biology , homology (biology) , biochemistry , conductance , plant cell , microbiology and biotechnology , chemistry , botany , gene , mathematics , combinatorics
Two cDNA clones encoding 14‐3‐3 homologous proteins were isolated from Vicia faba . Deduced amino acid sequences share different degrees of homology with other plant 14‐3‐3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14‐3‐3 proteins. These forms were mainly present in the microsomal fraction. Patch‐clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K + conductance compared to the wild type. This indicates the involvement of 14‐3‐3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel.