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Stromal and thylakoid‐bound ascorbate peroxidases are produced by alternative splicing in pumpkin 1
Author(s) -
Mano Shoji,
Yamaguchi Katsushi,
Hayashi Makoto,
Nishimura Mikio
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00862-4
Subject(s) - thylakoid , complementary dna , peroxidase , intron , microbiology and biotechnology , exon , biology , biochemistry , rna splicing , chloroplast , ascorbic acid , northern blot , rna , alternative splicing , gene , enzyme , food science
A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A) + RNA of pumpkin cotyledons by RT‐PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid‐bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spanning region located in the carboxy domain of tAPX. Southern blot hybridization and analysis of intron structure indicated that mRNAs for sAPX and tAPX, whose suborganellar localizations in chloroplasts are different, are produced by alternative splicing. Immunoblot analysis showed that the accumulation of sAPX and tAPX was differently regulated during germination and subsequent greening of pumpkin cotyledons.