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Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction
Author(s) -
Kawasaki Masato,
Satow Yoshinori,
Ohya Yoshikazu,
Anraku Yasuhiro
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00850-8
Subject(s) - protein splicing , intramolecular force , rna splicing , chemistry , autocatalysis , yeast , protein engineering , proteases , protease , protein folding , biochemistry , stereochemistry , enzyme , gene , catalysis , rna
Protein splicing is an autocatalytic reaction of a single polypeptide in which a spliced intervening sequence is excised out and the two external regions are ligated with the peptide bond to yield two mature proteins. We examined the reaction mechanism using a folding‐dependent in vitro protein splicing system. Protein splicing proceeds at an optimal pH of 7 and is an intramolecular reaction. The reaction is not inhibited by potential protease inhibitors, suggesting that its mechanism is different from those catalyzed by known proteases.