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Burdock , a novel retrotransposon in Drosophila melanogaster , integrates into the coding region of the cut locus
Author(s) -
Ponomarenko N.A.,
Bannikov V.M.,
Anashchenko V.A.,
Tchurikov N.A.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00844-2
Subject(s) - retrotransposon , open reading frame , locus (genetics) , transposable element , drosophila melanogaster , genetics , coding region , mutant , microbiology and biotechnology , biology , gene , peptide sequence
The burdock element is known to be the 2.6‐kb insertion into the same region of the cut locus in 12 independently obtained ct ‐lethal mutants. Here we have determined the complete sequences of this insertion and of the hot spot region. It was found that the burdock is a short retrotransposon with long terminal repeats and a single open reading frame (ORF). The polypeptide encoded by the burdock ORF contains two adjacent regions homologous to the gag and pol polyproteins of the gypsy mobile element. The burdock insertion interrupts the short ORF of the cut locus. The target site sequence of the burdock insertions is similar to the Drosophila topoisomerase II cleavage site.