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Mechanism of gluconate synthesis in Rhizobium meliloti by using in vivo NMR
Author(s) -
Portais Jean-Charles,
Tavernier Patricia,
Besson Isabelle,
Courtois Josiane,
Courtois Bernard,
Barbotin Jean-Noël
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00832-6
Subject(s) - lactone , rhizobium , chemistry , nuclear magnetic resonance spectroscopy , in vivo , dehydrogenation , stereochemistry , biochemistry , catalysis , biology , microbiology and biotechnology , gene
The dehydrogenation of [1‐ 13 C]‐ and [2‐ 13 C]glucose into gluconate was monitored by NMR spectroscopy in living cell suspensions of two Rhizobium meliloti strains. The synthesis of gluconate was accompanied, in the cellular environment, by the formation of two gluconolactones, a γ‐lactone being detected in addition to the expected δ‐lactone. These lactones – as well as the gluconate – could be further metabolized by the cells. The δ‐lactone was utilized faster than the γ‐lactone. The presence – in significant amounts – and the relative stability of the lactones raise the question of their possible physiological significance.