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9‐ cis ‐Retinoic acid enhances fatty acid‐induced expression of the liver fatty acid‐binding protein gene
Author(s) -
Poirier H,
Braissant O,
Niot I,
Wahli W,
Besnard P
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00830-2
Subject(s) - retinoic acid , retinoid x receptor , retinoic acid receptor , fatty acid binding protein , peroxisome , peroxisome proliferator activated receptor , free fatty acid receptor , oleic acid , microbiology and biotechnology , fatty acid , cytosol , gene expression , chemistry , receptor , messenger rna , biochemistry , biology , gene , nuclear receptor , transcription factor , enzyme , polyunsaturated fatty acid
The role of retinoic acids (RA) on liver fatty acid‐binding protein (L‐FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9‐ cis ‐Retinoic acid (9‐ cis ‐RA) specifically enhanced L‐FABP mRNA levels in a time‐ and dose‐dependent manner. The higher induction was found 6 h after addition of 10 −6 M 9‐ cis ‐RA in the medium. RA also enhanced further both L‐FABP mRNA levels and cytosolic L‐FABP protein content induced by oleic acid. The retinoid X receptor (RXR) and the peroxisome proliferator‐activated receptor (PPAR), which are known to be activated, respectively, by 9‐ cis ‐RA and long chain fatty acid (LCFA), co‐operated to bind specifically the peroxisome proliferator‐responsive element (PPRE) found upstream of the L‐FABP gene. Our result suggest that the PPAR‐RXR complex is the molecular target by which 9‐ cis ‐RA and LCFA regulate the L‐FABP gene.