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1,2‐Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes
Author(s) -
Yamamoto Yorihiro,
Kambayashi Yasuhiro,
Ito Takashi,
Nakano Minoru
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00823-5
Subject(s) - superoxide , chemistry , diacylglycerol kinase , chelerythrine , staurosporine , peroxynitrite , biochemistry , respiratory burst , protein kinase c , chemiluminescence , enzyme , organic chemistry
We examined the effect of 1‐palmitoyl‐2‐linoleoylglycerol (PLG), PLG hydroxide (PLG‐OH), and PLG hydroperoxide (PLG‐OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion‐sensitive reagent, 2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐ a ]pyrazin‐3‐one (MCLA). PLG‐OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PLG‐OH did not. 1,3‐Dilinoleoylglycerol hydroperoxide, 1‐palmitoyl‐2‐linoleoylphosphatidylcholine hydroperoxide, and linoleic acid hydroperoxide were much less efficient in stimulating human PMNs than PLG‐OOH. The PKC inhibitors, chelerythrine chloride and staurosporine, inhibited the stimulation of PMNs. Possible pathophysiological role of 1,2‐diacylglycerol hydroperoxides is discussed.