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Measurement of gluconeogenesis and pyruvate recycling in the rat liver: a simple analysis of glucose and glutamate isotopomers during metabolism of [1,2,3‐ 13 C 3 ]propionate
Author(s) -
Jones John G.,
Naidoo Randy,
Sherry A.Dean,
Jeffrey F.M.H.,
Cottam G.Larry,
Malloy Craig R.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00764-3
Subject(s) - gluconeogenesis , propionate , isotopomers , chemistry , citric acid cycle , biochemistry , endocrinology , metabolism , medicine , pyruvate carboxylase , pyruvic acid , biology , organic chemistry , enzyme , molecule
Simple equations that relate glucose and glutamate 13 C‐NMR multiplet areas to gluconeogenesis and pyruvate recycling during metabolism of [1,2,3‐ 13 C 3 ]propionate are presented. In isolated rat livers, gluconeogenic flux was 1.2 times TCA cycle flux and about 40% of the oxaloacetate pool underwent recycling to pyruvate prior to formation of glucose. The 13 C spectra of glucose collected from rats after gastric versus intravenous administration of [1,2,3‐ 13 C 3 ]propionate indicated that pyruvate recycling was slightly higher in vivo (49%) while glucose production was unchanged. This indicates that a direct measure of gluconeogenesis and pyruvate recycling may be obtained from a single 13 C‐NMR spectrum of blood collected after oral administration of enriched propionate.