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Cell cycle specificity of Fas‐mediated apoptosis in WIL‐2 cells
Author(s) -
Beletskaya Irina V,
Nikonova Larisa V,
Beletsky Igor P
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00740-0
Subject(s) - apoptosis , cytoplasm , fas ligand , fas receptor , microbiology and biotechnology , cell cycle , programmed cell death , antigen , cell , biology , chemistry , immunology , biochemistry
Antibodies to Fas/APO1 receptor induce effective apoptosis in WIL‐2 cells of the human B‐lymphoid line. Quantitative assessment of the extent of the death in cells synchronized by thymidine block revealed a significant increase in their sensitivity to the cytocidal effect mediated by Fas/APO1 during the G 1 phase of the cell cycle. Western analysis of the content of the p53 antigen in the cytoplasm and nuclei of the cells showed that the Fas/APO1‐induced death is accompanied by massive translocation of the p53 from the cytoplasm to the nucleus. These findings suggest that cell vulnerability to the Fas/APO1‐mediated apoptosis is subjected to regulation by cell cycle‐dependent mechanisms, one of which is probably the function of the p53 antigen.