Premium
RAP1‐like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors
Author(s) -
Simon Babette,
Wattler Frank,
Merchant Juanita L.,
Münch Karin,
Schütze Hans-Joachim,
Suske Guntram,
Arnold Rudolf
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00736-9
Subject(s) - transcription factor , sp1 transcription factor , rap1 , zinc finger , promoter , islet , transcription (linguistics) , sp3 transcription factor , gastrin , binding site , microbiology and biotechnology , yeast , dna binding protein , biology , chemistry , enhancer , genetics , biochemistry , gene , gene expression , signal transduction , endocrinology , diabetes mellitus , linguistics , philosophy , secretion
Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1‐ and Sp3‐mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.