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ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype
Author(s) -
Bouhedja W,
Sockalingum G.D,
Pina P,
Allouch P,
Bloy C,
Labia R,
Millot J.M,
Manfait M
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00725-4
Subject(s) - escherichia coli , attenuated total reflection , fourier transform infrared spectroscopy , strain (injury) , phenotype , microbiology and biotechnology , chemistry , point mutation , bacteria , nucleic acid , enterobacteriaceae , biology , mutant , genetics , biochemistry , infrared spectroscopy , gene , chemical engineering , organic chemistry , anatomy , engineering
Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM‐1, TEM‐2, TEM‐3, SHV‐2, SHV‐3, SHV‐4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM‐2 and SHV‐3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to β‐lactamase point‐mutations.