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High‐level expression of human c‐Src can cause a spherical morphology without loss of anchorage‐dependent growth of NIH 3T3 cells
Author(s) -
Kato Goro,
Maeda Shuichiro
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00722-9
Subject(s) - proto oncogene tyrosine protein kinase src , 3t3 cells , cell culture , microbiology and biotechnology , tyrosine kinase , cell growth , cell , chemistry , biology , phosphorylation , signal transduction , biochemistry , transfection , genetics
To investigate whether overexpression of human c‐Src leads to cell rounding in anchorage‐dependent NIH 3T3 fibroblasts, we have established c‐Src‐inducible cell lines using a lac repressor–operator system. RN1 cells, which expressed c‐Src at a high level after induction, exhibited a spherical morphology and ceased to grow in monolayer culture. RN1 cells, however, exhibited neither focus‐forming ability nor anchorage‐independent growth potential with or without induction. Induced RN1 c‐Src was phosphorylated at Ser 75 , a previously reported spherical cell‐associated site, and at Tyr 419 . These data demonstrated for the first time that highly elevated human c‐Src tyrosine kinase activity can cause NIH 3T3 cells to have a spherical morphology without loss of anchorage‐dependent growth. The inducible cell line should be useful to study the mechanism for cell rounding by c‐Src.