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Sense and antisense RNA for the membrane associated 40 kDa subunit M40 of the insect V‐ATPase
Author(s) -
Merzendorfer Hans,
Harvey William R,
Wieczorek Helmut
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00699-6
Subject(s) - sense (electronics) , rna , microbiology and biotechnology , biology , antisense rna , gene , polyadenylation , protein subunit , messenger rna , chemistry , genetics
A phagemid clone representing a polyadenylated antisense transcript was also isolated and sequenced. Using RT‐PCR, endogenous antisense RNA was detected in poly(A) RNA isolated from the larval midgut. Since Southern blots indicated a single gene locus, both the antisense RNA as well as the sense mRNA encoding subunit M40 seem to originate from the same gene.