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Genomic organization and functional characterization of the mouse GalR1 galanin receptor
Author(s) -
Wang Suke,
He Chaogang,
Maguire Maureen T,
Clemmons Anthony L,
Burrier Robert E,
Guzzi Mario F,
Strader Catherine D,
Parker Eric M,
Bayne Marvin L
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00695-9
Subject(s) - galanin , complementary dna , biology , galanin receptor , receptor , transmembrane domain , microbiology and biotechnology , exon , gene , coding region , genetics , neuropeptide
Galanin mediates diverse physiological functions in digestive, endocrine, and central nervous systems through G‐protein‐coupled receptors. Two galanin receptors have been cloned but the gene structures are unknown. We report genomic and cDNA cloning of the mouse GalR1 galanin receptor and demonstrate that the coding sequence is uniquely divided into three exons encoding the N‐terminal portion through the fifth transmebrane domain, the third intracellular loop, and the sixth transmembrane domain through the C‐terminus. Functional analysis of the encoded cDNA revealed active ligand binding and intracellular signaling. The expression is detected in brain, spinal cord, heart and skeletal muscle.