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Tissue‐specific expression of the human laminin α5‐chain, and mapping of the gene to human chromosome 20q13.2‐13.3 and to distal mouse chromosome 2 near the locus for the ragged ( Ra ) mutation
Author(s) -
Durkin Marian E.,
Loechel Frosty,
Mattei Marie-Geneviève,
Gilpin Brent J.,
Albrechtsen Reidar,
Wewer Ulla M.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00686-8
Subject(s) - biology , microbiology and biotechnology , locus (genetics) , gene mapping , complementary dna , gene map , gene , chromosome 7 (human) , northern blot , chromosomal region , genetics , chromosome
To investigate the function of the laminin α5‐chain, previously identified in mice, cDNA clones encoding the 953‐amino‐acid carboxy terminal G‐domain of the human laminin α5‐chain were characterized. Northern blot analysis showed that the laminin α5‐chain is expressed in human placenta, heart, lung, skeletal muscle, kidney, and pancreas. The human laminin α5‐chain gene ( LAMA5 ) was assigned to chromosome 20q13.2‐q13.3 by in situ hybridization, and the mouse gene ( Lama5 ) was mapped by linkage analysis to a syntonic region of distal chromosome 2, close to the locus for the ragged ( Ra ) mutation.