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Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen
Author(s) -
Armour Kenneth J.,
Lehane David B.,
Pakdel Farzad,
Valotaire Yves,
Russell R.Graham G.,
Henderson Ian W.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00680-7
Subject(s) - rainbow trout , estrogen receptor , estrogen , chemistry , messenger rna , medicine , bone mineral , endocrinology , blot , trout , in vivo , biology , osteoporosis , biochemistry , gene , fish <actinopterygii> , fishery , genetics , cancer , breast cancer
RT‐PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss . The rainbow trout estrogen receptor (ER)‐specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32 P‐labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.