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Immunological identification of the alternative oxidase of Acanthamoeba castellanii mitochondria
Author(s) -
Jarmuszkiewicz Wieslawa,
Wagner Anneke M,
Wagner Marijke J,
Hryniewiecka Lilla
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00676-5
Subject(s) - alternative oxidase , acanthamoeba , biochemistry , oxidase test , mitochondrion , protozoa , biology , monoclonal antibody , amoeba (genus) , cyanide , enzyme , microbiology and biotechnology , chemistry , antibody , immunology , inorganic chemistry
Mitochondria of the protozoa Acanthamoeba castellanii possess a cyanide‐insensitive oxidase cross‐reacting with monoclonal antibodies raised against the plant alternative oxidase. Immunoblotting revealed three monomeric forms (38, 35, and 32 kDa) and very low amounts of a single 65 kDa dimeric form. Cross‐linking studies suggest that while in plants the alternative oxidase occurs as a dimer, in amoeba it functions as a monomer. Immunologically detectable protein levels change with the age of amoeba cell culture. Increased amounts of the 35 kDa protein are accompanied by an increase in the activity of cyanide‐resistant respiration.