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The pH‐dependent reciprocal changes in contributions of ADP/ATP antiporter and aspartate/glutamate antiporter to the fatty acid‐induced uncoupling
Author(s) -
Samartsev Victor N,
Mokhova Ele,
Skulachev Vladimir P
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00667-4
Subject(s) - antiporter , glutamate receptor , chemistry , mitochondrion , glutamate aspartate transporter , biochemistry , stimulation , biophysics , respiration , membrane , metabotropic glutamate receptor , biology , endocrinology , receptor , anatomy
The pH effect on carboxyatractylate‐, glutamate‐ and aspartate‐induced recoupling of palmitate‐uncoupled rat liver mitochondria has been studied. Stimulation of respiration by low palmitate concentrations (5–20 μM) in the presence of 3 mM MgCl 2 is shown to be pH‐independent within the 7.0–7.8 range. The recoupling effect of glutamate (or aspartate) decreases and that of carboxyatractylate increases with increase in pH. The recoupling effect of a combination of carboxyatractylate and glutamate (aspartate) appears to be constant at these pH values, being as high as about 80%. It is concluded that uncoupling by low palmitate in liver mitochondria is mediated mainly by ATP/ADP and aspartate/glutamate antiporter.